Synthesis and Operating Optimization of the PEG Conjugate via CuAAC in scCO₂

A new sustainable green protocol for obtaining polyethylene glycol (PEG) conjugates, with a prototype molecule, which in this work was coumarin, by means of click chemistry is presented. The organic solvents commonly used type reaction were replaced supercritical carbon dioxide (scCO2). synthesis and characterization PEG-coumarin successfully reported using FTIR, 1H NMR, MALDI TOF. Subsequently, preliminary study carried out the response surface methodology to examine variables that most affect use scCO2 as medium. main effects caused these variables, individually their binary interaction, have been estimated. has screen factorial design 23. p-values temperature pressure 0.006 0.0117, being therefore significant study. more intensive on shown greatest effect performance where an 82.32% success achieved, broadens scope conjugated coumarin mPEG-alkyne evaluated vitro antioxidant activities DPPH radical scavenging assay found exhibit substantial activities. product showed comparable or even better efficacy than initial coumarin.